human monocytic aml cells thp 1  (ATCC)

Journal: Antibody Therapeutics

Article Title: Fc gamma receptors promote antibody-induced LILRB4 internalization and immune regulation of monocytic AML

doi: 10.1093/abt/tbad025

Figure Lengend Snippet: FcγR-dependent and independent mechanisms of h128-3-induced LILRB4 internalization on monocytic AML. Depending on the monocytic AML cell surface levels of functional FcγRs, h128-3 binds and internalizes its LILRB4 target receptor on these malignant cells by three potential mechanisms: (1) on all monocytic AML cells, h128-3 crosslinking of LILRB4 receptors triggers clathrin-mediated endocytosis and lysosomal receptor degradation; (2) on FcγR high monocytic AML cells, high-affinity FcγRI scaffolds h128-3 to target LILRB4, leading to internalization and degradation; and (3) on FcγRI − FcγRIIA + monocytic AML cells, low-affinity FcγRIIA scaffolds h128-3 by avidity to target LILRB4, prompting its internalization and degradation.

Article Snippet: Human monocytic AML cell line Mono-mac-6 was obtained from DSMZ and maintained in a humidified atmosphere of 5% CO 2 at 37°C, in RPMI supplemented with 10% FBS (HyClone), 1 mM sodium pyruvate, non-essential amino acids (Thermo Fisher), 10 μg/ml human insulin (Sigma) and 100 U/ml penicillin and 100 μg/ml streptomycin (Life Technologies).

Techniques: Functional Assay

Journal: Antibody Therapeutics

Article Title: Fc gamma receptors promote antibody-induced LILRB4 internalization and immune regulation of monocytic AML

doi: 10.1093/abt/tbad025

Figure Lengend Snippet: FcγR-dependent and independent mechanisms of h128-3-induced LILRB4 internalization on monocytic AML. Depending on the monocytic AML cell surface levels of functional FcγRs, h128-3 binds and internalizes its LILRB4 target receptor on these malignant cells by three potential mechanisms: (1) on all monocytic AML cells, h128-3 crosslinking of LILRB4 receptors triggers clathrin-mediated endocytosis and lysosomal receptor degradation; (2) on FcγR high monocytic AML cells, high-affinity FcγRI scaffolds h128-3 to target LILRB4, leading to internalization and degradation; and (3) on FcγRI − FcγRIIA + monocytic AML cells, low-affinity FcγRIIA scaffolds h128-3 by avidity to target LILRB4, prompting its internalization and degradation.

Article Snippet: Human monocytic AML cell line THP-1 was obtained from ATCC and maintained in a humidified atmosphere of 5% CO 2 at 37°C, in R10 [RPMI supplemented with 10% FBS (HyClone) and 100 U/ml penicillin and 100 μg/ml streptomycin (Life Technologies)].

Techniques: Functional Assay